Journal archive > 2005 > N 3, May-June

The effect of buthionine sulfoximine on the glutathione level in goldfish tissues

V. I. Lushchak, M. Hermes-Lima

This study describes the effect of DL-buthionine-[S,R]-sulfoximine (BSO) on the glutathione equivalents (GSH-eq = GSH + 2 GSSG) of goldfish. BSO causes depletion of cellular GSH by inhibiting ?-glutamylcysteine synthetase, a key enzyme of the GSH biosynthesis pathway. BSO at 1,000 and 1,500 mg/kg was effective in promoting 50 and 80% depletion of GSH-eq from brain and liver, respectively, within 3 days. Lower doses of BSO failed to effectively promote hepatic GSH-eq depletion. Moreover, no evident toxic side-effects were observed (including hepatic lipid peroxidation and free radical-mediated oxidation of proteins) in goldfish in response to BSO intraperitoneal injections. We conclude that BSO can be used to deplete GSH eq in goldfish liver and brain, but attention should be paid to species-specific variations in BSO effects.

Recieved: 2005-01-13

Published at the site: 2005-05-05

The original article in English is available for download in PDF format.

 

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