Journal archive > 2005 > N2, March-April

Influence of caffeine and cyclosporin A on the transmembrane exchange of Ca2+ in smooth muscle mitochondria

O. B. Vadzyuk 

The effects of cyclosporin A and caffeine on the active and passive transport of Са2+ in mitochondria isolated from adult rat myometrium were studied by fluorescent technique using Са2+-sensitive probe tetracycline (TC). It was shown that 5 µM cyclosporin increases Са2+ accumulation by the mitochondria matrix. But it fails to exhibit such effect when 20 mM caffeine was also present in the incubation medium, while the inhibitory action of caffeine on the accumulation of Са2+ reveals nevertheless in the absence or presence of cyclosporin A. In case of the preliminary incubation of mitochondria with 10 mM caffeine before the initiation of transport process one could also observe the inhibition of kinetic parameters of the active accumulation of Са2+ by the mitochondria. It was also shown, that caffeine stimulates passive efflux of Са2+ from the myometrium mitochondria. Thus we conclude, that the stimulating effect of cyclosporin on Са2+ accumulation by the myometrium mitochondria is sensitive to caffeine, while caffeine has no direct effect on Са2+-uniporter, but it evidently disturbs the barrier function of the inner mitochondria membrane in such way, that stimulating effect of cyclosporin A cannot develop.

Recieved: 2004-11-12

Published at the site: 2005-03-03

The original article in Ukrainian is available for download in PDF format.

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