Journal archive > 2005 > N 2, March-April

Novel and simple ELISA-based method for antibody affinity determination

S. A. Bobrovnik, S. V. Komisarenko, L. V. Ilyina

New coordinates for antibody affinity determination by ELISA are suggested. The suggested approach is very simple but at the same time it is more convenient and is deprived of the drawbacks inhered in the earlier suggested methods. The examples of antibody affinity determination by the suggested methods for both simulative and experimental binding curves are considered. It was demonstrated that the suggested methods allow getting more precise values of antibody affinity.

Recieved: 2004-11-12

Published at the site: 2005-03-03

The original article in English is available for download in PDF format.