Metabolism of С2-С6-substrates under mixotrophic growth of Acinetobacter sp. В-7005 and B 7005 (1НГ) strains
Activities of the key enzymes of С2-С6-metabolism were assayed under cultivation of Acinetobacter sp. В-7005 and B-7005 (1НГ) strains on ethanol and glucose mixture. Under mixotrophic growth of bacteria the enzymes activity of ethanol metabolism (NAD+-dependent alcohol dehydrogenase, NADP+-dependent acetaldehyde dehydrogenase, acetyl-KoA-synthetase) and glucose metabolism (6-phosphofructokinase and 6-phosphogluconate dehydratase) was lower than that on corresponding monosubstrates. The activity of isocitrate lyase and malate synthase in cells grown on the substrate mixture declined to an even greater extent, indicating that the role of the glyoxylate cycle in such cells is insignificant. The simultaneous functioning of the glyoxylate cycle and pyruvate carboxylase reaction, increasing of phosphoenolpyruvate synthetase activity testify to the gluconeogenesis intensification under mixotrophic growth of Acinetobacter sp. В-7005 and B-7005 (1НГ).
Published at the site: 2004-02-02
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