Journal archive > 2004 > N 6 November-December

Quantification of D-dimer and soluble fibrin in blood plasma at ischemic heart disease and hypertension

E. V. Lugovskoy, I. N. Kolesnikova, N. E. Lugovskaya, L. M. Litvinova,
P. G. Gritsenko, G. K. Gogolinskaya, E. D. Lyashko, E. P. Kostyuchenko,
G. A. Remisovsky, V. N. Pedchenko, S. V. Komisarenko

The method of D-dimer quantification in the human blood plasma has been developed using monoclonal antibodies III-3b and II-4d. The method has been verified on the blood plasma of the patients with ischemic heart disease with and without stenocardia and with hypertension. The results showed that at ischemic heart disease with and without stenocardia and at hypertension the quantities of D-dimer in the blood plasma were generally less than the highest normal level 500 ng/ml (64.3%, 76.2% and 95%, correspondingly). The semiquantitative measurements of soluble fibrin levels in blood plasmas of the patients with ischemic heart disease and hypertension have been performed. It has been shown that the quantity of soluble fibrin at these diseases range greatly from < 0.03 mg/ml to 0.15 mg/ml. There was no correlation between the quantities of D-dimer and soluble fibrin in blood plasmas of the patients. Electrophoresis in PAAG with SDS showed that the soluble fibrin at these diseases had the molecular mass of the fibrin (ogen). Thus the soluble fibrin in blood plasmas analysed consisted mainly of fibrin desAA oligomers (may be with fibrinogen incorporation) which are not stabilized by the factor XIIIa.

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