New approach to an increase in specificity of determination of antibodies to lipopolysaccharides
of gram-negative bacteria by solid-phase enzyme-linked immunosorbent assay
A simple and effective method is proposed to increase the sorption of lipopolysaccharides (LPS) of Gram-negative bacteria on the polystyrene surfaces, in which there is no need of preliminary modification of the surface of the solid phase with poly-L-lysine. The method consists in using 0.05 M carbonate buffer (pH 9.6) with 70% of ammonium sulfate at the stage of LPS immobilization. As a result, LPS absorption increases essentially, and the level of non-specific reactions remains low. This makes
the usage of additional blocking reagents unnecessary and allows increasing sensitivity and specificity in determination of anti-LPS-antibodies by the solid-phase enzyme-linked immunosorbent assay.
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