Journal archive > 2004 > N 5, September-October

Specific and nonspecific interactions of antibodies and immunoglobulines with antigens
and the methods of analysis of these interactions

S. A. Bobrovnik

The problem of specific and nonspecific interaction of serum immunoglobulins and antigens was considered. It was shown that high-sensitive methods allow to reveal low-affinity non-specific interaction of immunoglobulins and antigens. If the concentration of the specific antibodies in a studied sample of serum is low, the non-specific interaction of serum immunoglobulins may exceed substantially the effect of specific reaction. In this case the obtained results could be misinterpreted. In this connection the conclusion has been done that in such a case it is necessary to take into account the capability of serum immunoglobulins to interact non-specifically with antigens and to discriminate between specific and non-specific interaction. The methods of the diminishing the non-specific interaction are suggested.

Recieved: 2004-06-25

Published at the site: 2004-10-10

The original article in Russian is available for download in PDF format.