Thrombospondin-1-dependent mechanism of transforming growth factor ?-1
activation in cultured human endothelial cells
Using two immortal human umbilical vein endothelial-derived cell lines we investigated the thrombospondin-1 (TSP-1)–dependent mechanism of the transforming growth factor ?-1 (TGF-1) activation. Exogenous human platelet TSP-1 was demonstrated to increase (1.7–2.0-folds) the concentration of active TGF?1 produced by both cell lines, but did not influence the concentration of total TGF?1. The process of TGFb1 activation is known to be regulated by G418GWSHW423 fragment of TSP-1 molecule. The simultaneous addition of TSP-1 and 100-fold molar excess (about TSP-1) of the synthetic GGWSHW peptide abolished TSP-1-induced TGFB1-1 activation.
GGWSHW peptide inhibited the basal activation of TGF?1 in endothelial cells, whereas the secretion of total TGF?1 and TSP-1 was not altered. We conclude that TSP-1-dependent mechanism of endogenous TGF?1 activation is operation in human endothelial cells.
Published at the site: 2004-10-10
The original article in Ukrainian is available for download in PDF format.