Influence of cadmium chloride and hydrogen peroxide on the content of phosphoinositides in isolated hepatocytes of rats
Influence of cadmium chloride and hydrogen peroxide on the processes of lipid peroxidation and contents of polyphosphoinositides in isolated hepatocytes of rats was studied. It is shown that incubation of the cells with cadmium chloride or hydrogen peroxide already in 15 min results in the reinforcement of lipid peroxidation and increase of contents of phosphatidylinositol and phosphatidylinositoldiphosphate . Variations of the basal level of the latter, as is well known, plays an important messenger role. In case of more long incubation (60 min) one can observe the significant accumulation of intermediate and final products of lipid peroxidation, while the content of phosphatidylinositol and phosphatidylinositoldiphosphate did not differ from the control, but the content of phosphatidylinositolphosphate was increased. Changes in the content of separate fraction of polyphosphoinositides in the presence of cadmium chloride and hydrogen peroxide were similar. Allowing for all that it is, possible to draw a conclusion that the mechanism of cadmium influence on the content of polyphosphoinositides is based on its ability to cause development of the oxidative stress.
Published at the site: 2004-06-06
The original article in Russian is available for download in PDF format.