Journal archive > 2004 > N3, May-June

Isolation and characteristics of the protein C activator from Agkistrodon halys halys snake venom

T. M. Platonova, O. V. Gornitskaya

Protein C activator from Agkistrodon halys halys venom has been isolated and purified by ion-exchange and gel-filtration chromatography. The purified enzyme consists of the single peptide-chain with molecular weight of 34 kDa. Its pH-optimum was in 7,5–8,0 range. The enzyme was inhibited by DFP, benzamidine, PMSF, EGTA. Protein C activator was as effective as “Protac” (Pentapharm AG, Switzerland).

Recieved: 2003-06-19

Published at the site: 2004-06-06

The original article in Russian is available for download in PDF format.