Influence of ?-radiation on human erythrocyte surface potential under eicosanoid metabolism inhibition
The aim of the research consisted in the study of influence of ?-radiation on response of erythrocyte surface potential to inhibitors of eicosanoid metabolism enzymes (cyclo-, lipoxygenase and phospholipase A2).
It was shown, that inhibitors of phospholipase А2 (quinacrine, 10–100 ?M), cyclooxygenase (aspirin, 10–100 ?M) and cyclo- and lipoxygenase (BW755c, 1–100 ?M) lowered electrophoretic mobility (EPM) of erythrocytes by 20–30%. An analogous effect can be exerted by ?-radiation. Nonradioactive leucine in the studied concentrations cannot simulate EPM erythrocytes. Response of cellular EPM to these inhibitors depended on their concentration in the incubation medium. Addition of 14C to the incubation medium changed response of EPM of cells to inhibitors of cyclo- and lipoxygenase but not to quinacrine. However ?-radiation fully abolished the stimulative action of nonspecific activator of phospholipase А2 (Ca-independent), Н2О2, on cellular EPM. Under these conditions ?-radiation enhanced EPM response to aspirin only at concentration of 100 muM. The EPM response to BW755c is reduced by irradiation at all concentrations with the exception of equal-effective one (10 ?M).
Data obtained evidence for modification of eicosanoid metabolism by ?-radiation, probably, as a result of phospholipase А2 inhibition, as evident from elimination by radiation of stimulated action of hydrogen peroxide on EPM. The radiation action can also affect the cyclooxygenase lipoxygenase activity ratio, this activity being mediated by cellular membrane signaling systems.
Published at the site: 2004-04-04
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