Activity of mem?brane Na+, K+-ATPase of embryos of loach (Misgurnus fossilis L.) under the influence of heavy metal cations
The activity of membrane Nа+, K+-АТPase of embryos of loach Misgurnus fossilis L. at early stages of development in the normal conditions and under the influence of heavy metal cations has been investigated. It was established, that the influence of such heavy metal cations as Ni2+, Co2+, Sn2+, Zn2+, Mn2+ and Cd2+ in concentrations 10-6 –10-4 М results in reduction of activity of membrane Nа+, K+-АТPase of loach embryos. It was shown, that the inhibition effect is more expressed with the increase of concentrations of heavy metal cations in the incubation medium. The definition of inhibition constants I0.5 has allowed to analyze the sensitivity of Nа+, K+-АТPase to influence of various cations of heavy metals at different stages of blastomer division. Possible mechanisms of influence of heavy metal cations on the activity of membrane Nа+, K+-АТPase of loach embryos have been considered.
Published at the site: 2004-04-0
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